Cloned (Comment) | Organism |
---|---|
expression of His-tagged XMT1 in Escherichia coli strain BL21(DE3) | Coffea canephora |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM xanthosine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.0 A resolution | Coffea canephora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, about, sequence calculation | Coffea canephora |
80000 | - |
about, recombinant enzyme, gel filtration | Coffea canephora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + xanthosine | Coffea canephora | - |
S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea canephora | A4GE69 | XMT1; var. Robusta | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration | Coffea canephora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + xanthosine | - |
Coffea canephora | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, about, sequence calculation | Coffea canephora |
Synonyms | Comment | Organism |
---|---|---|
XMT1 | - |
Coffea canephora |